Plasmid

Part:BBa_M11400:Design

Designed by: Cody Tramp   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2010-04-24)


Integration Plasmid for Synechocystis sp. PCC 6803


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1336
    Illegal XbaI site found at 1321
    Illegal SpeI site found at 1306
    Illegal PstI site found at 1292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1336
    Illegal NheI site found at 1537
    Illegal NheI site found at 5952
    Illegal SpeI site found at 1306
    Illegal PstI site found at 1292
    Illegal NotI site found at 1119
    Illegal NotI site found at 1297
    Illegal NotI site found at 1313
    Illegal NotI site found at 1328
    Illegal NotI site found at 1548
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1336
    Illegal BglII site found at 2706
    Illegal BamHI site found at 2917
    Illegal BamHI site found at 5272
    Illegal XhoI site found at 30
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1336
    Illegal XbaI site found at 1321
    Illegal SpeI site found at 1306
    Illegal PstI site found at 1292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1336
    Illegal XbaI site found at 1321
    Illegal SpeI site found at 1306
    Illegal PstI site found at 1292
    Illegal NgoMIV site found at 2041
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4247
    Illegal BsaI.rc site found at 2156


Design Notes

Site-directed mutagenesis of EcoRI site between RS1 and kanamycin resistance gene. Removal of GFP expression cassette and T7 terminator. Addition of translation and transcription stop sequences flanking BioBrick prefix and suffix sites (from BioBrick Basic Vector BBa_I51020).


Source

Genomic Sequence, Genbank

References